Plant Transcription Factor Database
Previous version: v3.0
Cannabis sativa
HSF Family
Species TF ID Description
PK02428.1HSF family protein
PK02473.1HSF family protein
PK02473.3HSF family protein
PK02473.4HSF family protein
PK03562.1HSF family protein
PK04832.1HSF family protein
PK05866.1HSF family protein
PK05866.2HSF family protein
PK09831.1HSF family protein
PK09831.2HSF family protein
PK11988.1HSF family protein
PK12075.1HSF family protein
PK12544.1HSF family protein
PK14700.2HSF family protein
PK14700.3HSF family protein
PK14700.4HSF family protein
PK15309.1HSF family protein
PK17549.1HSF family protein
PK19243.1HSF family protein
PK19243.2HSF family protein
PK20762.1HSF family protein
PK22028.1HSF family protein
PK22356.1HSF family protein
PK25112.1HSF family protein
PK26221.1HSF family protein
PK26417.1HSF family protein
HSF Family Introduction

Heat stress transcription factors (Hsfs) are the major regulators of the plant heat stress (hs) response. Sequencing of the Arabidopsis genome revealed the existence of 21 open-reading frames (ORFs) encoding putative Hsfs assigned to classes A-C. Here we present results of a functional genomics approach to the Arabidopsis Hsf family focused on the analysis of their C-terminal domains (CTDs) harboring conserved modules for their function as transcription factors and their intracellular localization. Using reporter assays in tobacco protoplasts and yeast as well as glutathione-S-transferase (GST) pull-down assays, we demonstrate that short peptide motifs enriched with aromatic and large hydrophobic amino acid (aa) residues embedded in an acidic surrounding (AHA motifs) are essential for transcriptional activity of class A Hsfs. In contrast to this, class B and C Hsfs lack AHA motifs and have no activator function on their own. We also provide evidence for the function of a leucine (Leu)-rich region centered around a conserved QMGΦL motif at the very C-terminus as a nuclear export signal (NES) of class A Hsfs. Sequence comparison indicates that the combination of a C-terminal AHA motif with the consensus sequence FWxxF/L,F/I/L as well as the adjacent NES represents a signature domain for plant class A Hsfs, which allowed to identify more than 60 new Hsfs from the expressed sequence tag (EST) database.

Kotak S, Port M, Ganguli A, Bicker F, von Koskull-Doring P.
Characterization of C-terminal domains of Arabidopsis heat stress transcription factors (Hsfs) and identification of a new signature combination of plant class A Hsfs with AHA and NES motifs essential for activator function and intracellular localization.
Plant J, 2004. 39(1): p. 98-112.
PMID: 15200645